Description
Priciple of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGFbeta2. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated TGFbeta2. A competitive inhibition reaction is launched between TGFbeta2 (Standards or samples) and Biotin-conjugated TGFbeta2 with the pre-coated antibody specific for TGFbeta2. The more amount of TGFbeta2 in samples, the less antibody bound by Biotin-conjugated TGFbeta2. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of TGFbeta2 in the sample. The color development is stopped and the intensity of the color is measured